They said: "This could mean thousands of healthy badgers dying across Oxfordshire and Berkshire, where culling under licence is legally allowed.
"We know that there are proposals to issue licences which would see culling starting in new areas of Berkshire and Oxfordshire later this year."
Each culling licence lasts for 4 years.
The current consultation is also requesting an extension to these licences - meaning badger culling would continue until at least 2026 or even 2028, decimating many badger populations.
The Wildlife Trust said:
"We’ve asked the Government to stop issuing culling licences IMMEDIATELY and we’re asking you to do so too. Please respond to the Government’s consultation and add your voice to the growing calls to stop the cull today."
You can sign their petition by clicking here.The consultation ends on 24th March.
The Trust are instead advocating that farmers vaccinate their cattle for TB, which they believe would be a more effective way of curbing TB spread.