The iconic music venue has launched a change.org petition to help persuade the Bucks County Museum Trust to stage an exhibition to celebrate the landmark anniversary.
Friars Aylesbury opened in Aylesbury on 2 June 1969 and will be celebrating its 50th anniversary in 2019.
David Stopps, from Friars said: "Aylesbury is generally known for four things, its ducks, Stoke Mandeville Paraplegic Games, its music club Friars Aylesbury and Earthly Messenger, the world's first statue of David Bowie.
"Friars has been in communication with the Bucks County Museum Director for over a year, about creating a proper exhibition to mark this important cultural milestone in Aylesbury.
history, but has finally been told no.
"Please support this petition to persuade the Bucks County Museum Trust to help Buckinghamshire celebrate this important and historic event!"
The Bucks County Museum held an exhibition back in 2013, which was very popular.
Back in 2013, the exhibition included audio and visual elements that covered 43 years of Aylesbury events and gigs Friars presented in Princes Risborough, Bedford, Watford, Dunstable and High Wycombe including those featuring The Faces, Elton John, Genesis, Pink Floyd and David Bowie.
To sign the petition, please visit:
A statement from Bucks County Museum said: "Bucks County Museum Trust is an independent Charitable Trust. Our mission is to welcome the involvement of the wide range of communities within Buckinghamshire and we welcome considered proposals for sponsored exhibitions, which will be popular within the community.
"We are naturally keen to support FRIARS; indeed, we held a major exhibition celebrating its history in 2014. However, when we received a request to hold an exhibition in 2019 on Friars, we explained that we plan our exhibitions programme well in advance because of the time it takes to curate and design each exhibition, and that we already had in place a firmed up complete programme for 2019.
"We also have major plans to redevelop the Story of Bucks permanent galleries over the next couple of years and are making applications for significant funds to make this a reality. When work goes ahead, not only will it will cause considerable disruption to our exhibition programme, but our staff will need to focus all their time on developing the plans for the new displays.
"Nevertheless, we are keen to hear the outcome of the petition, which will give an indication of demand, and if that was very strong and the proposed exhibition has full sponsorship, we would consider holding an exhibition in 2020 or some future date.
"We have already offered our wonderful garden facility and hospitality spaces for events as a venue for the anniversary celebrations as an expression of our support for the Friars 50 th Anniversary."
Friars Aylesbury has presented some of the biggest bands in the world in Aylesbury including: The Ramones, The Clash, David Bowie, U2, Genesis, The Jam, Madness, The Specials,
Motorhead, Erasure, Ian Dury, Iggy Pop, Blondie, The Police, Marillion and Pink Floyd amongst hundreds of others.
Friars will be presenting several concerts in 2019 to mark its 50th
Anniversary, including The Vaccines (24 Jan), Nick Mason's Saucerful of Secrets (with Nick Mason & Gary Kemp playing the early music of Pink Floyd - 30 April) and Steve Hackett (2 Nov).