HS2 Ltd's application to drive on the A413/A355 corridor from north of Wendover to the M40 at Beaconsfield has been declined.
Bucks Council denied the measure on the grounds that the applications lacked information and concerns over the impact HS2's large vehicles would have on the roads.
HS2 have appealed against the Council’s refusal to allow the applications and it will now be up to the Secretary of State for Transport to make the decision, or he may allocate the decision to the Planning Inspectorate.
Martin Tett, Bucks Council leader said: “Buckinghamshire Council has consistently opposed the building of HS2. Not only do we doubt the strategic case for it, but we also recognised the negative impact it would have on our local communities up and down the line.
"Now that construction has started we are seeing the devastating environmental damage to our beautiful county and we are doing our best to hold HS2 to account."
Council Leader Tett, further outlined the reasons the council oppose the use of these lorries across Buckinghamshire, adding: “The use of the A413/A355 for hundreds of heavy lorry movements a day for several years is a massive issue for Buckinghamshire.
"Before considering lorry route applications, the Council has to ensure that the cumulative impact of the additional HS2 HGVs on the A413/A355 will not bring Buckinghamshire to a standstill. HS2 have not provided us with the information we require to give us any reassurance on this issue."
A spokesperson for the council cited refusal of the public body to share information from an assessment that was carried out as a key issue.
The spokesperson said in a statement: "Since the HS2 Act was approved, HS2 Ltd and its contractors have changed the way that the railway is to be constructed.
"One of these changes combines lorry routes onto the A413/A355 corridor. Although HS2 Ltd have provided some information on the numbers of anticipated HGVs and proposes a number of improvements, the Council does not consider that it has sufficient information to demonstrate that the changes would not have a significant impact on the free flow of traffic, particularly given that this single route would be used for all work sites south of Aylesbury as far as Amersham."
Council Leader Tett further explained the damaging effect he believes these lorries could have on the environment around Aylesbury, he said: "“The lorries have the potential to impact not only on highway traffic but also the local environment in terms of noise and pollution and also on all other road users.
"It is our responsibility to help ensure that the effects of that construction traffic is minimised as much as possible. The proposed routes will affect communities and residents from north of Wendover all the way to Beaconsfield and Buckinghamshire Council cannot and will not approve these applications on this basis, and HS2 has been informed of our position.”