The Waterside Whittington Walk team have been making final preparations ahead of their 52 mile challenge from Aylesbury to London.
Waitrose has supplied the team with water, bananas and high energy snacks, and other companies including The Works, Chiltern Railways and Motts Travel have all donated to the cause.
The group, consisting of staff from the theatre have taken their name from this year’s pantomime Dick Whittington.
They will be setting off at 4am and hope to raise £5,000 for the Florence Nightingale Hospice’s children team.
To donate visit www.justgiving.com/watersidewhittingtonwalkers